ICAM-1 expression measured using our FRET-based nanosensor, on live MDA-MB-231 breast cancer cells
Spatiotemporal sensing of cell surface protein expression can be useful in studying cell behavior in various disease phenotypes. We are currently exploiting Förster Resonance Energy Transfer (FRET) between fluorophores and gold nanoparticles (AuNPs) to develop nanobiosensors for real-time sensing using fluorescence.
One of the cell receptor densities we want to measure is ICAM-1. ICAM-1 has been correlated with a multitude of disease phenotypes, including cancer initiation, progression and metastasis, polarization of tumor associated neutrophils and neutrophils in the context of sepsis, to name a few.